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Gatan K3 direct electron detector positioned post a Gatan BioQuantum energy filter (Gatan, Pleasanton, California, USA), operated in a zero-loss mode with a slit width of 25 eV was used to acquire dose fractionated images of the samples with a 100 µm objective aperture
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Advances in interventional cardiac surgeries have significantly improved the repair and management of CHD, resulting in an adult population with a higher prevalence of CHD [2]